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cd22 expression plasmid  (Sangon Biotech)

 
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    Sangon Biotech cd22 expression plasmid
    Expression of miR-19a and <t>CD22</t> in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.
    Cd22 Expression Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd22+expression+plasmid/pmc04459571-64-1-6?v=Sangon+Biotech
    Average 90 stars, based on 1 article reviews
    cd22 expression plasmid - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis"

    Article Title: MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.894321

    Expression of miR-19a and CD22 in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.
    Figure Legend Snippet: Expression of miR-19a and CD22 in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.

    Techniques Used: Expressing, Infection, Control, Comparison, Fluorescence, Flow Cytometry

    Expressions of miR-19a and CD22 in activated B cells. PBMCs obtained from healthy volunteers were activated by LPS, and B cells were isolated after activation for the detection of miR-19a. The relative expression levels of miR-19a determined by qRT-PCR was increased by 2 days and 4 days in activated B cells compared to those in control B cells without stimulus ( A ). The mean fluorescence intensity (MFI) of CD22 on CD19 gated PBMCs determined by flow cytometry were increased by 2 days but decreased by 4 days ( B ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05.
    Figure Legend Snippet: Expressions of miR-19a and CD22 in activated B cells. PBMCs obtained from healthy volunteers were activated by LPS, and B cells were isolated after activation for the detection of miR-19a. The relative expression levels of miR-19a determined by qRT-PCR was increased by 2 days and 4 days in activated B cells compared to those in control B cells without stimulus ( A ). The mean fluorescence intensity (MFI) of CD22 on CD19 gated PBMCs determined by flow cytometry were increased by 2 days but decreased by 4 days ( B ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05.

    Techniques Used: Isolation, Activation Assay, Expressing, Quantitative RT-PCR, Control, Fluorescence, Flow Cytometry

    Function of miR-19a in B cell activation. PBMCs transfected with miR-19a mimic or inhibitor (anti-miR19a), or corresponding controls (miR-NC, anti-miR-NC) were activated by LPS for 48 h. Activated B cells transfected with miR-19a mimic exhibited a higher level of p-BLNK, which was measured by Western blotting, while inhibition of miR-19a resulted in a suppressed level of p-BLNK ( A ). Overexpression of miR-19a induced decreases in CD22 mRNA while inhibition of miR-19a resulted in increased levels of CD22 mRNA ( B ). B cells transfected with miR-19a exhibited down-regulated CD22 expression, and inhibition of miR-19a enhanced CD22 expression ( C ). The effect of miR-19a mimic/inhibitor transfection was confirmed by the detection of miR-19a using qRT-PCR ( D ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms and protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.
    Figure Legend Snippet: Function of miR-19a in B cell activation. PBMCs transfected with miR-19a mimic or inhibitor (anti-miR19a), or corresponding controls (miR-NC, anti-miR-NC) were activated by LPS for 48 h. Activated B cells transfected with miR-19a mimic exhibited a higher level of p-BLNK, which was measured by Western blotting, while inhibition of miR-19a resulted in a suppressed level of p-BLNK ( A ). Overexpression of miR-19a induced decreases in CD22 mRNA while inhibition of miR-19a resulted in increased levels of CD22 mRNA ( B ). B cells transfected with miR-19a exhibited down-regulated CD22 expression, and inhibition of miR-19a enhanced CD22 expression ( C ). The effect of miR-19a mimic/inhibitor transfection was confirmed by the detection of miR-19a using qRT-PCR ( D ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms and protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Techniques Used: Activation Assay, Transfection, Western Blot, Inhibition, Over Expression, Expressing, Quantitative RT-PCR

    Influence of CD22 overexpression on miR-19a. PBMCs were co-transfected with CD22 expression plasmid (pc-CD22) or control (pc-DNA), and with miR-19a mimic or control (miR-NC), and were activated by LPS for 48 h. Western blotting revealed that CD22 overexpression partially attenuated the up-regulation of p-BLNK induced by miR-19a transfection ( A ). qRT-PCR analysis demonstrated that overexpression of CD22 resulted in an increased level of miR-19a in activated B cells ( B ). The effect of CD22 plasmid was confirmed by the detection of CD22 mRNA using qRT-PCR ( C ). Data are expressed as mean ±SEM of 3 independent experiments. Protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.
    Figure Legend Snippet: Influence of CD22 overexpression on miR-19a. PBMCs were co-transfected with CD22 expression plasmid (pc-CD22) or control (pc-DNA), and with miR-19a mimic or control (miR-NC), and were activated by LPS for 48 h. Western blotting revealed that CD22 overexpression partially attenuated the up-regulation of p-BLNK induced by miR-19a transfection ( A ). qRT-PCR analysis demonstrated that overexpression of CD22 resulted in an increased level of miR-19a in activated B cells ( B ). The effect of CD22 plasmid was confirmed by the detection of CD22 mRNA using qRT-PCR ( C ). Data are expressed as mean ±SEM of 3 independent experiments. Protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Techniques Used: Over Expression, Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Quantitative RT-PCR



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    cd22 expression plasmid  (Sangon Biotech)
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    Sangon Biotech cd22 expression plasmid
    Expression of miR-19a and <t>CD22</t> in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.
    Cd22 Expression Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd22+expression+plasmid/pmc04459571-64-1-6?v=Sangon+Biotech
    Average 90 stars, based on 1 article reviews
    cd22 expression plasmid - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of miR-19a and CD22 in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis

    doi: 10.12659/MSM.894321

    Figure Lengend Snippet: Expression of miR-19a and CD22 in B cells obtained from patients and controls. The relative expression levels of miR-19a in patients with sepsis (sepsis group) and in patients with non-infected SIRS (SIRS group) were significantly higher than that in healthy controls (control group), and the level in the sepsis group was elevated in comparison to that in the SIRS group ( A ). The levels of CD22 expression were displayed as geometrical mean fluorescence intensity (Geom. MFI) measured by flow cytometry, and were of no differences among different groups ( B ). Data are expressed as mean ±SD. * P<0.05, ** P<0.01.

    Article Snippet: The CD22 expression plasmid (provided by Sango Biotech, China) was generated by amplification of the entire coding region of CD22.

    Techniques: Expressing, Infection, Control, Comparison, Fluorescence, Flow Cytometry

    Expressions of miR-19a and CD22 in activated B cells. PBMCs obtained from healthy volunteers were activated by LPS, and B cells were isolated after activation for the detection of miR-19a. The relative expression levels of miR-19a determined by qRT-PCR was increased by 2 days and 4 days in activated B cells compared to those in control B cells without stimulus ( A ). The mean fluorescence intensity (MFI) of CD22 on CD19 gated PBMCs determined by flow cytometry were increased by 2 days but decreased by 4 days ( B ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis

    doi: 10.12659/MSM.894321

    Figure Lengend Snippet: Expressions of miR-19a and CD22 in activated B cells. PBMCs obtained from healthy volunteers were activated by LPS, and B cells were isolated after activation for the detection of miR-19a. The relative expression levels of miR-19a determined by qRT-PCR was increased by 2 days and 4 days in activated B cells compared to those in control B cells without stimulus ( A ). The mean fluorescence intensity (MFI) of CD22 on CD19 gated PBMCs determined by flow cytometry were increased by 2 days but decreased by 4 days ( B ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05.

    Article Snippet: The CD22 expression plasmid (provided by Sango Biotech, China) was generated by amplification of the entire coding region of CD22.

    Techniques: Isolation, Activation Assay, Expressing, Quantitative RT-PCR, Control, Fluorescence, Flow Cytometry

    Function of miR-19a in B cell activation. PBMCs transfected with miR-19a mimic or inhibitor (anti-miR19a), or corresponding controls (miR-NC, anti-miR-NC) were activated by LPS for 48 h. Activated B cells transfected with miR-19a mimic exhibited a higher level of p-BLNK, which was measured by Western blotting, while inhibition of miR-19a resulted in a suppressed level of p-BLNK ( A ). Overexpression of miR-19a induced decreases in CD22 mRNA while inhibition of miR-19a resulted in increased levels of CD22 mRNA ( B ). B cells transfected with miR-19a exhibited down-regulated CD22 expression, and inhibition of miR-19a enhanced CD22 expression ( C ). The effect of miR-19a mimic/inhibitor transfection was confirmed by the detection of miR-19a using qRT-PCR ( D ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms and protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis

    doi: 10.12659/MSM.894321

    Figure Lengend Snippet: Function of miR-19a in B cell activation. PBMCs transfected with miR-19a mimic or inhibitor (anti-miR19a), or corresponding controls (miR-NC, anti-miR-NC) were activated by LPS for 48 h. Activated B cells transfected with miR-19a mimic exhibited a higher level of p-BLNK, which was measured by Western blotting, while inhibition of miR-19a resulted in a suppressed level of p-BLNK ( A ). Overexpression of miR-19a induced decreases in CD22 mRNA while inhibition of miR-19a resulted in increased levels of CD22 mRNA ( B ). B cells transfected with miR-19a exhibited down-regulated CD22 expression, and inhibition of miR-19a enhanced CD22 expression ( C ). The effect of miR-19a mimic/inhibitor transfection was confirmed by the detection of miR-19a using qRT-PCR ( D ). Data are expressed as mean ±SEM of 3 independent experiments. Histograms and protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Article Snippet: The CD22 expression plasmid (provided by Sango Biotech, China) was generated by amplification of the entire coding region of CD22.

    Techniques: Activation Assay, Transfection, Western Blot, Inhibition, Over Expression, Expressing, Quantitative RT-PCR

    Influence of CD22 overexpression on miR-19a. PBMCs were co-transfected with CD22 expression plasmid (pc-CD22) or control (pc-DNA), and with miR-19a mimic or control (miR-NC), and were activated by LPS for 48 h. Western blotting revealed that CD22 overexpression partially attenuated the up-regulation of p-BLNK induced by miR-19a transfection ( A ). qRT-PCR analysis demonstrated that overexpression of CD22 resulted in an increased level of miR-19a in activated B cells ( B ). The effect of CD22 plasmid was confirmed by the detection of CD22 mRNA using qRT-PCR ( C ). Data are expressed as mean ±SEM of 3 independent experiments. Protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis

    doi: 10.12659/MSM.894321

    Figure Lengend Snippet: Influence of CD22 overexpression on miR-19a. PBMCs were co-transfected with CD22 expression plasmid (pc-CD22) or control (pc-DNA), and with miR-19a mimic or control (miR-NC), and were activated by LPS for 48 h. Western blotting revealed that CD22 overexpression partially attenuated the up-regulation of p-BLNK induced by miR-19a transfection ( A ). qRT-PCR analysis demonstrated that overexpression of CD22 resulted in an increased level of miR-19a in activated B cells ( B ). The effect of CD22 plasmid was confirmed by the detection of CD22 mRNA using qRT-PCR ( C ). Data are expressed as mean ±SEM of 3 independent experiments. Protein bands were obtained from 1 of 3 independent experiments that displayed similar results. * P<0.05, ** P<0.01.

    Article Snippet: The CD22 expression plasmid (provided by Sango Biotech, China) was generated by amplification of the entire coding region of CD22.

    Techniques: Over Expression, Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Quantitative RT-PCR